PPY (1-96) 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPY (1-96) partial fragment
Host
Human
Theoretical MW (kDa)
10.67
Interspecies Antigen Sequence
Mouse (74); Rat (77)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPY (1-96) antibody (H00005539-B02) by Western Blots.
SDS-PAGE Gel
PPY transfected lysate.
Western Blot
Lane 1: PPY transfected lysate ( 10.56 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPY
Entrez GeneID
5539GeneBank Accession#
BC032225Protein Accession#
AAH32225Gene Name
PPY
Gene Alias
PNP
Gene Description
pancreatic polypeptide
Omim ID
167780Gene Ontology
HyperlinkGene Summary
This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. [provided by RefSeq
Other Designations
pancreatic polypeptide Y
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Interactome
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