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Last updated: 2016/10/23
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PPP2R5C monoclonal antibody (M01), clone 3G9

  • Catalog # : H00005527-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant PPP2R5C.
  • Immunogen:
  • PPP2R5C (NP_002710.2, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of PPP2R5C expression in transfected 293T cell line by PPP2R5C monoclonal antibody (M01), clone 3G9.

    Lane 1: PPP2R5C transfected lysate(61.1 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged PPP2R5C is approximately 3ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Proximity Ligation Analysis of protein-protein interactions between TP53 and PPP2R5C. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PPP2R5C mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5527
  • Gene Name:
  • PPP2R5C
  • Gene Alias:
  • B56G,MGC23064,PR61G
  • Gene Description:
  • protein phosphatase 2, regulatory subunit B', gamma isoform
  • Gene Summary:
  • The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • B' alpha regulatory subunit,PP2A, B subunit, B' gamma isoform,PP2A, B subunit, B56 gamma isoform,PP2A, B subunit, PR61 gamma isoform,PP2A, B subunit, R5 gamma isoform,Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, gamma isoform,gamma
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