PPP2R1A monoclonal antibody (M02), clone 4E6
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More Files
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Western Blot (Cell lysate)
PPP2R1A monoclonal antibody (M02), clone 4E6. Western Blot analysis of PPP2R1A expression in Jurkat(Cat # L017V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPP2R1A is 3 ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TP53 and PPP2R1A. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PPP2R1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PPP2R1A.
Immunogen
PPP2R1A (AAH01537, 1 a.a. ~ 589 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFATLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PPP2R1A monoclonal antibody (M02), clone 4E6. Western Blot analysis of PPP2R1A expression in Jurkat(Cat # L017V1 ).Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPP2R1A is 3 ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TP53 and PPP2R1A. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PPP2R1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — PPP2R1A
Entrez GeneID
5518GeneBank Accession#
BC001537Protein Accession#
AAH01537Gene Name
PPP2R1A
Gene Alias
MGC786, PR65A
Gene Description
protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
Omim ID
605983Gene Ontology
HyperlinkGene Summary
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq
Other Designations
PP2A, subunit A, PR65-alpha isoform|PP2A, subunit A, R1-alpha isoform|alpha isoform of regulatory subunit A, protein phosphatase 2|medium tumor antigen-associated 61 KDA protein|protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isof
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Interactome
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Pathway
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Disease
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