PPOX 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PPOX transfected lysate.
Western Blot
Lane 1: PPOX transfected lysate ( 50.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPOX full-length
Host
Human
Theoretical MW (kDa)
50.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPOX antibody (H00005498-B01) by Western Blots.
SDS-PAGE Gel
PPOX transfected lysate.
Western Blot
Lane 1: PPOX transfected lysate ( 50.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPOX
Entrez GeneID
5498GeneBank Accession#
NM_000309Protein Accession#
NP_000300Gene Name
PPOX
Gene Alias
MGC8485, PPO, V290M, VP
Gene Description
protoporphyrinogen oxidase
Gene Ontology
HyperlinkGene Summary
This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000032237|OTTHUMP00000032238|variegate porphyria
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