PPOX monoclonal antibody (M04A), clone 2B5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PPOX expression in transfected 293T cell line by PPOX monoclonal antibody (M04A), clone 2B5.
Lane 1: PPOX transfected lysate (Predicted MW: 50.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PPOX.
Immunogen
PPOX (AAH02357, 378 a.a. ~ 477 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EASGCVLSQELFQQRAQEAAATQLGLKEMPSHCLVHLHKNCIPQYTLGHWQKLESARQFLTAHRLPLTLAGASYEGVAVNDCIESGRQAAVSVLGTEPNS
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In ascites fluid
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PPOX expression in transfected 293T cell line by PPOX monoclonal antibody (M04A), clone 2B5.
Lane 1: PPOX transfected lysate (Predicted MW: 50.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — PPOX
Entrez GeneID
5498GeneBank Accession#
BC002357Protein Accession#
AAH02357Gene Name
PPOX
Gene Alias
MGC8485, PPO, V290M, VP
Gene Description
protoporphyrinogen oxidase
Gene Ontology
HyperlinkGene Summary
This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000032237|OTTHUMP00000032238|variegate porphyria
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Interactome
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Pathway
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