PPM1G 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: PPM1G transfected lysate ( 59.3 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
PPM1G transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPM1G full-length
Host
Human
Theoretical MW (kDa)
60.17
Interspecies Antigen Sequence
Mouse (92); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPM1G antibody (H00005496-B01) by Western Blots.
Western Blot
Lane 1: PPM1G transfected lysate ( 59.3 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
PPM1G transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPM1G
Entrez GeneID
5496GeneBank Accession#
NM_002707Protein Accession#
NP_002698Gene Name
PPM1G
Gene Alias
MGC1675, MGC2870, PP2CG, PP2CGAMMA, PPP2CG
Gene Description
protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Omim ID
605119Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq
Other Designations
OTTHUMP00000123426|PP2C, gamma|protein phosphatase 1C|protein phosphatase 1G|protein phosphatase 2, catalytic subunit, gamma isoform|protein phosphatase 2C gamma isoform|protein phosphatase magnesium-dependent 1 gamma
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Interactome
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Disease
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