PPM1B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
PPM1B transfected lysate.
Western Blot
Lane 1: PPM1B transfected lysate ( 52.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPM1B full-length
Host
Human
Theoretical MW (kDa)
52.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPM1B antibody (H00005495-B01) by Western Blots.
SDS-PAGE Gel
PPM1B transfected lysate.
Western Blot
Lane 1: PPM1B transfected lysate ( 52.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPM1B
Entrez GeneID
5495GeneBank Accession#
NM_002706.4Protein Accession#
-Gene Name
PPM1B
Gene Alias
MGC21657, PP2C-beta-X, PP2CB, PP2CBETA, PPC2BETAX
Gene Description
protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Omim ID
603770Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq
Other Designations
OTTHUMP00000158953|OTTHUMP00000158955|protein phosphatase 1B|protein phosphatase 2C beta isoform|protein phosphatase 2C-like protein
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Interactome
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Pathway
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Disease
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