PPIA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PPIA transfected lysate.
Western Blot
Lane 1: PPIA transfected lysate ( 18 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPIA full-length
Host
Human
Theoretical MW (kDa)
18
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPIAantibody (H00005478-D01P)byWesternBlots.
SDS-PAGE Gel
PPIA transfected lysate.
Western Blot
Lane 1: PPIA transfected lysate ( 18 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPIA
Entrez GeneID
5478GeneBank Accession#
NM_021130Protein Accession#
NP_066953.1Gene Name
PPIA
Gene Alias
CYPA, CYPH, MGC117158, MGC12404, MGC23397
Gene Description
peptidylprolyl isomerase A (cyclophilin A)
Omim ID
123840Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000025305|PPIase A|T cell cyclophilin|cyclosporin A-binding protein|peptidyl-prolyl cis-trans isomerase A|peptidylprolyl isomerase A|rotamase A
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Interactome
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Disease
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