PPEF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PPEF1 transfected lysate.
Western Blot
Lane 1: PPEF1 transfected lysate ( 75.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPEF1 full-length
Host
Human
Theoretical MW (kDa)
75.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPEF1 antibody (H00005475-D01P) by Western Blots.
SDS-PAGE Gel
PPEF1 transfected lysate.
Western Blot
Lane 1: PPEF1 transfected lysate ( 75.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPEF1
Entrez GeneID
5475GeneBank Accession#
NM_006240.2Protein Accession#
NP_006231.2Gene Name
PPEF1
Gene Alias
PP7, PPEF, PPP7C
Gene Description
protein phosphatase, EF-hand calcium binding domain 1
Omim ID
300109Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq
Other Designations
OTTHUMP00000023006|protein phosphatase with EF hand calcium-binding domain 1|protein phosphatase, EF hand calcium-binding domain 1|protein phosphatase, serine/threonine type, with EF-hands|serine/threonine protein phosphatase 7
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