PPBP monoclonal antibody (M01), clone 3B9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.
Lane 1: PPBP transfected lysate(13.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPBP is approximately 0.03ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi ( Cat # H00005473-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 (Cat # H00005473-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (35.75 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PPBP.
Immunogen
PPBP (AAH28217, 37 a.a. ~ 128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TKGQTKRNLAKGKEESLDSDLYAELRCMCIKTTSGIHPKNIQSLEVIGKGTHCNQVEVIATLKDGRKICLDPDAPRIKKIVQKKLAGDESAD
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PPBP expression in transfected 293T cell line by PPBP monoclonal antibody (M01), clone 3B9.
Lane 1: PPBP transfected lysate(13.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPBP is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PPBP over-expressed 293 cell line, cotransfected with PPBP Validated Chimera RNAi ( Cat # H00005473-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPBP monoclonal antibody (M01), clone 3B9 (Cat # H00005473-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — PPBP
Entrez GeneID
5473GeneBank Accession#
BC028217Protein Accession#
AAH28217Gene Name
PPBP
Gene Alias
B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1
Gene Description
pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
Omim ID
121010Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. [provided by RefSeq
Other Designations
CXC chemokine ligand 7|beta-thromboglobulin|connective tissue-activating peptide III|leukocyte-derived growth factor|low-affinity platelet factor IV|macrophage-derived growth factor|neutrophil-activating peptide 2|neutrophil-activating peptide-2|platelet
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Interactome
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Pathway
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Disease
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