PPAT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PPAT transfected lysate.
Western Blot
Lane 1: PPAT transfected lysate ( 56.98 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPAT full-length
Host
Human
Theoretical MW (kDa)
56.98
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPAT antibody (H00005471-B01) by Western Blots.
SDS-PAGE Gel
PPAT transfected lysate.
Western Blot
Lane 1: PPAT transfected lysate ( 56.98 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPAT
Entrez GeneID
5471GeneBank Accession#
NM_002703.3Protein Accession#
NP_002694.3Gene Name
PPAT
Gene Alias
ATASE, GPAT, PRAT
Gene Description
phosphoribosyl pyrophosphate amidotransferase
Omim ID
172450Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. [provided by RefSeq
Other Designations
amidophosphoribosyltransferase|glutamine PRPP amidotransferase|glutamine phosphoribosylpyrophosphatate amidotransferase
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Interactome
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Pathway
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