PPARA 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPARA full-length
Host
Human
Theoretical MW (kDa)
52.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CACNG3 antibody (H00005465-D01P) by Western Blots.
SDS-PAGE Gel
PPARA transfected lysate.
Western Blot
Lane 1: PPARA transfected lysate ( 52.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPARA
Entrez GeneID
5465GeneBank Accession#
NM_001001928Protein Accession#
NP_001001928.1Gene Name
PPARA
Gene Alias
MGC2237, MGC2452, NR1C1, PPAR, hPPAR
Gene Description
peroxisome proliferator-activated receptor alpha
Omim ID
170998Gene Ontology
HyperlinkGene Summary
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq
Other Designations
OTTHUMP00000028713|OTTHUMP00000042872|peroxisome proliferative activated receptor, alpha|peroxisome proliferator activated receptor alpha
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