POU4F1 monoclonal antibody (M04), clone 7B4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant POU4F1.
Immunogen
POU4F1 (NP_006228.2, 331 a.a. ~ 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QAWLEEAEGAQREKMNKPELFNGGEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.
Lane 1: POU4F1 transfected lysate(46.09 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.ELISA
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Gene Info — POU4F1
Entrez GeneID
5457GeneBank Accession#
NM_006237Protein Accession#
NP_006228.2Gene Name
POU4F1
Gene Alias
BRN3A, FLJ13449, Oct-T1, RDC-1
Gene Description
POU class 4 homeobox 1
Omim ID
601632Gene Ontology
HyperlinkGene Summary
BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T-lymphocytic lineages (Gerrero et al., 1993 [PubMed 8248179]).[supplied by OMIM
Other Designations
OTTHUMP00000018535|POU domain, class 4, transcription factor 1
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