POR 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
POR transfected lysate.
Western Blot
Lane 1: POR transfected lysate ( 74.91 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POR full-length
Host
Human
Theoretical MW (kDa)
74.91
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POR antibody (H00005447-B01) by Western Blots.
SDS-PAGE Gel
POR transfected lysate.
Western Blot
Lane 1: POR transfected lysate ( 74.91 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POR
Entrez GeneID
5447GeneBank Accession#
BC034277.1Protein Accession#
AAH34277.1Gene Name
POR
Gene Alias
CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R
Gene Description
P450 (cytochrome) oxidoreductase
Gene Ontology
HyperlinkGene Summary
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq
Other Designations
NADPH-dependent cytochrome P450 reductase|cytochrome P450 reductase
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