POLRMT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
POLRMT transfected lysate.
Western Blot
Lane 1: POLRMT transfected lysate ( 138.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POLRMT full-length
Host
Human
Theoretical MW (kDa)
138.5
Interspecies Antigen Sequence
Mouse (66); Rat (65)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POLRMT antibody (H00005442-B01) by Western Blots.
SDS-PAGE Gel
POLRMT transfected lysate.
Western Blot
Lane 1: POLRMT transfected lysate ( 138.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POLRMT
Entrez GeneID
5442GeneBank Accession#
BC098387Protein Accession#
AAH98387Gene Name
POLRMT
Gene Alias
APOLMT, MTRNAP, MTRPOL, h-mtRPOL
Gene Description
polymerase (RNA) mitochondrial (DNA directed)
Omim ID
601778Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq
Other Designations
mitochondrial DNA-directed RNA polymerase
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Interactome
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