POLR2I monoclonal antibody (M01), clone 2B3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant POLR2I.
Immunogen
POLR2I (AAH17112, 26 a.a. ~ 125 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged POLR2I is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — POLR2I
Entrez GeneID
5438GeneBank Accession#
BC017112Protein Accession#
AAH17112Gene Name
POLR2I
Gene Alias
RPB9, hRPB14.5
Gene Description
polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Omim ID
180662Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq
Other Designations
DNA directed RNA polymerase II polypeptide I|polymerase (RNA) II (DNA directed) polypeptide I (14.5kD)
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Interactome
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Pathway
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