POLR2F monoclonal antibody (M02), clone 2G2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of POLR2F expression in transfected 293T cell line by POLR2F monoclonal antibody (M02), clone 2G2.
Lane 1: POLR2F transfected lysate (Predicted MW: 14.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged POLR2F is 0.3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to POLR2F on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (40.9 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant POLR2F.
Immunogen
POLR2F (NP_068809.1, 1 a.a. ~ 127 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIITD
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (40.9 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of POLR2F expression in transfected 293T cell line by POLR2F monoclonal antibody (M02), clone 2G2.
Lane 1: POLR2F transfected lysate (Predicted MW: 14.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged POLR2F is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to POLR2F on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — POLR2F
Entrez GeneID
5435GeneBank Accession#
NM_021974.2Protein Accession#
NP_068809.1Gene Name
POLR2F
Gene Alias
HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6
Gene Description
polymerase (RNA) II (DNA directed) polypeptide F
Omim ID
604414Gene Ontology
HyperlinkGene Summary
This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. [provided by RefSeq
Other Designations
DNA directed RNA polymerase II 14.4 kda polypeptide|DNA directed RNA polymerase II polypeptide F|RNA Polymerase II subunit 14.4 kD
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Interactome
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Pathway
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Disease
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