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POLR2E 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005434-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-POLR2E full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 23.21
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-POLR2E antibody (H00005434-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005434-T01
    POLR2E transfected lysate.
    Western Blot
    QC Testing of H00005434-T01
    Lane 1: POLR2E transfected lysate ( 23.21 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5434
  • Gene Name:
  • POLR2E
  • Gene Alias:
  • RPABC1,RPB5,XAP4,hRPB25,hsRPB5
  • Gene Description:
  • polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
  • Gene Summary:
  • This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq
  • Other Designations:
  • DNA directed RNA polymerase II 23 kda polypeptide,DNA directed RNA polymerase II polypeptide E,polymerase (RNA) II (DNA directed) polypeptide E (25kD)
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