POLR2E rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human POLR2E peptide using ARM Technology.
Immunogen
A synthetic peptide of human POLR2E is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human POLR2E peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — POLR2E
Entrez GeneID
5434GeneBank Accession#
POLR2EGene Name
POLR2E
Gene Alias
RPABC1, RPB5, XAP4, hRPB25, hsRPB5
Gene Description
polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
Omim ID
180664Gene Ontology
HyperlinkGene Summary
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq
Other Designations
DNA directed RNA polymerase II 23 kda polypeptide|DNA directed RNA polymerase II polypeptide E|polymerase (RNA) II (DNA directed) polypeptide E (25kD)
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