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POLR2E rabbit monoclonal antibody

  • Catalog # : H00005434-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human POLR2E peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human POLR2E is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human POLR2E peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • Application Image
  • Western Blot (Transfected lysate)
  • Gene Information
  • Entrez GeneID:
  • 5434
  • Gene Name:
  • POLR2E
  • Gene Alias:
  • RPABC1,RPB5,XAP4,hRPB25,hsRPB5
  • Gene Description:
  • polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
  • Gene Summary:
  • This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq
  • Other Designations:
  • DNA directed RNA polymerase II 23 kda polypeptide,DNA directed RNA polymerase II polypeptide E,polymerase (RNA) II (DNA directed) polypeptide E (25kD)
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