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POLH 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005429-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-POLH full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 78.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-POLH antibody (H00005429-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005429-T01
    POLH transfected lysate.
    Western Blot
    QC Testing of H00005429-T01
    Lane 1: POLH transfected lysate ( 78.4 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5429
  • Protein Accession#:
  • -
  • Gene Name:
  • POLH
  • Gene Alias:
  • FLJ16395,FLJ21978,RAD30,RAD30A,XP-V,XPV
  • Gene Description:
  • polymerase (DNA directed), eta
  • Gene Summary:
  • This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000016478,OTTHUMP00000039868
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