PNMT 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PNMT full-length
Host
Human
Theoretical MW (kDa)
31.13
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PNMT antibody (H00005409-B01) by Western Blots.
Western Blot
Lane 1: PNMT transfected lysate ( 30.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
PNMT transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PNMT
Entrez GeneID
5409GeneBank Accession#
NM_002686.2Protein Accession#
-Gene Name
PNMT
Gene Alias
MGC34570, PENT, PNMTase
Gene Description
phenylethanolamine N-methyltransferase
Omim ID
171190Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq
Other Designations
OTTHUMP00000164271|noradrenaline N-methyltransferase|phenylethanolamine N-methylase
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Interactome
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Pathway
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Disease
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