PMM2 monoclonal antibody (M01), clone 2E9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PMM2 expression in transfected 293T cell line by PMM2 monoclonal antibody (M01), clone 2E9.
Lane 1: PMM2 transfected lysate(28.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PMM2 is 0.1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (32.89 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PMM2.
Immunogen
PMM2 (NP_000294, 47 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCRQNIQSHLGEALIQDLINYCLSYIAKIK
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (32.89 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PMM2 expression in transfected 293T cell line by PMM2 monoclonal antibody (M01), clone 2E9.
Lane 1: PMM2 transfected lysate(28.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PMM2 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — PMM2
Entrez GeneID
5373GeneBank Accession#
NM_000303Protein Accession#
NP_000294Gene Name
PMM2
Gene Alias
CDG1, CDG1a, CDGS
Gene Description
phosphomannomutase 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Mutations in this gene have been shown to cause defects in glycoprotein biosynthesis, which manifests as carbohydrate-deficient glycoprotein syndrome type I. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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