PLTP monoclonal antibody (M01), clone 2F3-G4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PLTP.
Immunogen
PLTP (AAH05045, 19 a.a. ~ 441 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
FPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGHFYYNISEVKVTELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFLKVYDFLSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (72.05 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PLTP monoclonal antibody (M01), clone 2F3-G4 Western Blot analysis of PLTP expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of PLTP expression in transfected 293T cell line by PLTP monoclonal antibody (M01), clone 2F3-G4.
Lane 1: PLTP transfected lysate(54.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PLTP on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLTP is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — PLTP
Entrez GeneID
5360GeneBank Accession#
BC005045Protein Accession#
AAH05045Gene Name
PLTP
Gene Alias
HDLCQ9
Gene Description
phospholipid transfer protein
Omim ID
172425Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000031678|lipid transfer protein II
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Interactome
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Pathway
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Disease
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Publication Reference
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PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity.
Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, Pais de Barros JP, Desrumaux C, Combe B, Hahne M, Lagrost L, Morel J.
PLoS One 2018 Mar; 13(3):e0193815.
Application:Blocking, Func, Recombinant proteins.
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Increased Biglycan in Aortic Valve Stenosis Leads to the Overexpression of Phospholipid Transfer Protein via Toll-Like Receptor 2.
Derbali H, Bosse Y, Cote N, Pibarot P, Audet A, Pepin A, Arsenault B, Couture C, Despres JP, Mathieu P.
The American Journal of Pathology 2010 Jun; 176(6):2638.
Application:IHC-Fr, Human, Aortic valves.
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PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity.
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