PLS3 monoclonal antibody (M01), clone 5B9
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
PLS3 monoclonal antibody (M01), clone 5B9. Western Blot analysis of PLS3 expression in MCF-7.
Western Blot (Cell lysate)
PLS3 monoclonal antibody (M01), clone 5B9. Western Blot analysis of PLS3 expression in MDA-MB-231.
Western Blot (Transfected lysate)
Western Blot analysis of PLS3 expression in transfected 293T cell line by PLS3 monoclonal antibody (M01), clone 5B9.
Lane 1: PLS3 transfected lysate (Predicted MW: 70.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLS3 is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PLS3 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (94.93 KDa) .
-
Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PLS3.
Immunogen
PLS3 (AAH39049, 1 a.a. ~ 630 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDEMATTQISKDELDELKEAFAKVDLNSNGFICDYELHELFKEANMPLPGYKVREIIQKLMLDGDRNKDGKISFDEFVYIFQEVKSSDIAKTFRKAINRKEGICALGGTSELSSEGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTDDLFKAVGDGIVLCKMINLSVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCHVVNIGAEDLRAGKPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEELLLRWANFHLENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRIDINMSGFNETDDLKRAESMLQQADKLGCRQFVTPADVVSGNPKLNLAFVANLFNKYPALTKPENQDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLYADLQDALVILQLYERIKVPVDWSKVNKPPYPKLGANMKKLENCNYAVELGKHPAKFSLVGIGGQDLNDGNQTLTLALVWQLMRRYTLNVLEDLGDGQKANDDIIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLVKSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMGRGMKRV
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (94.93 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Cell lysate)
PLS3 monoclonal antibody (M01), clone 5B9. Western Blot analysis of PLS3 expression in MCF-7.Western Blot (Cell lysate)
PLS3 monoclonal antibody (M01), clone 5B9. Western Blot analysis of PLS3 expression in MDA-MB-231.Western Blot (Transfected lysate)
Western Blot analysis of PLS3 expression in transfected 293T cell line by PLS3 monoclonal antibody (M01), clone 5B9.
Lane 1: PLS3 transfected lysate (Predicted MW: 70.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLS3 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PLS3 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PLS3
Entrez GeneID
5358GeneBank Accession#
BC039049.1Protein Accession#
AAH39049Gene Name
PLS3
Gene Alias
T-plastin
Gene Description
plastin 3 (T isoform)
Omim ID
300131Gene Ontology
HyperlinkGene Summary
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
T isoform|T plastin|plastin 3
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com