PLOD1 rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human PLOD1 peptide using ARM Technology.
Immunogen
A synthetic peptide of human PLOD1 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human PLOD1 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — PLOD1
Entrez GeneID
5351GeneBank Accession#
PLOD1Gene Name
PLOD1
Gene Alias
FLJ42041, LH, LH1, LLH, PLOD
Gene Description
procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
Gene Ontology
HyperlinkGene Summary
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. [provided by RefSeq
Other Designations
OTTHUMP00000002512|lysine hydroxylase|lysyl hydroxylase|lysyl hydroxylase 1|procollagen-lysine, 2-oxoglutarate 5-dioxygenase
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