PLK1 monoclonal antibody (M01), clone 2G12
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
PLK1 monoclonal antibody (M01), clone 2G12 Western Blot analysis of PLK1 expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Cell lysate)
Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.
Lane 1: PLK1 transfected lysate(68.3 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (91.85 KDa) .
-
Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PLK1.
Immunogen
PLK1 (AAH02369, 1 a.a. ~ 603 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (91.85 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Cell lysate)
PLK1 monoclonal antibody (M01), clone 2G12 Western Blot analysis of PLK1 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Cell lysate)
Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.
Lane 1: PLK1 transfected lysate(68.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — PLK1
Entrez GeneID
5347GeneBank Accession#
BC002369Protein Accession#
AAH02369Gene Name
PLK1
Gene Alias
PLK, STPK13
Gene Description
polo-like kinase 1 (Drosophila)
Omim ID
602098Gene Ontology
HyperlinkOther Designations
cell cycle regulated protein kinase|polo (Drosophia)-like kinase|polo like kinase|polo-like kinase
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com