PLD1 monoclonal antibody (M01), clone 2F3
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PLD1.
Immunogen
PLD1 (NP_002653, 965 a.a. ~ 1074 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PLD1 expression in transfected 293T cell line by PLD1 monoclonal antibody (M01), clone 2F3.
Lane 1: PLD1 transfected lysate(124.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLD1 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — PLD1
Entrez GeneID
5337GeneBank Accession#
NM_002662Protein Accession#
NP_002653Gene Name
PLD1
Gene Alias
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Gene Description
phospholipase D1, phosphatidylcholine-specific
Omim ID
602382Gene Ontology
HyperlinkGene Summary
Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM
Other Designations
choline phosphatase 1|phospholipase D1, phophatidylcholine-specific
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Interactome
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Pathway
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Disease
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Publication Reference
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Mechanisms for the activity of heterocyclic cyclohexanone curcumin derivatives in estrogen receptor negative human breast cancer cell lines.
Somers-Edgar TJ, Taurin S, Larsen L, Chandramouli A, Nelson MA, Rosengren RJ.
Investigational New Drugs 2011 Feb; 29(1):87.
Application:WB-Ce, Human, MDA-MB-231, SKBr3 cells.
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Mechanisms for the activity of heterocyclic cyclohexanone curcumin derivatives in estrogen receptor negative human breast cancer cell lines.
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