PLCG2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PLCG2 full-length
Host
Human
Theoretical MW (kDa)
147.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PLCG2 antibody (H00005336-D01P) by Western Blots.
SDS-PAGE Gel
PLCG2 transfected lysate.
Western Blot
Lane 1: PLCG2 transfected lysate ( 147.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PLCG2
Entrez GeneID
5336GeneBank Accession#
BC007565Protein Accession#
AAH07565.1Gene Name
PLCG2
Gene Alias
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Gene Description
phospholipase C, gamma 2 (phosphatidylinositol-specific)
Omim ID
600220Gene Ontology
HyperlinkGene Summary
Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide-specific phospholipase C enzymes, C-beta is regulated by heterotrimeric G protein-coupled receptors, while the closely related C-gamma-1 (PLCG1; MIM 172420) and C-gamma-2 enzymes are controlled by receptor tyrosine kinases. The C-gamma-1 and C-gamma-2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.[supplied by OMIM
Other Designations
phospholipase C gamma 2|phospholipase C, gamma 2|phospholipase C, gamma 2 (phosphatidylyinositol-specific)
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Interactome
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Pathway
- B cell receptor signaling pathway
- Calcium signaling pathway
- Epithelial cell signaling in Helicobacter pylori infection
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
- Fc gamma R-mediated phagocytosis
- Glioma
- Inositol phosphate metabolism
- Leukocyte transendothelial migration
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Disease
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