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Last updated: 2016/12/4
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PKLR 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005313-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PKLR full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 63.25
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PKLR antibody (H00005313-B01) by Western Blots.
    Western Blot
    QC Testing of H00005313-T01
    Lane 1: PKLR transfected lysate ( 61.8 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00005313-T01
    PKLR transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5313
  • Protein Accession#:
  • -
  • Gene Name:
  • PKLR
  • Gene Alias:
  • PK1,PKL,PKR,PKRL,RPK
  • Gene Description:
  • pyruvate kinase, liver and RBC
  • Gene Summary:
  • The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • R-type/L-type pyruvate kinase,pyruvate kinase 1,pyruvate kinase isozyme R/L,pyruvate kinase type L,pyruvate kinase, liver and RBC type,pyruvate kinase, liver and blood cell,red cell/liver pyruvate kinase
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