PKLR 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: PKLR transfected lysate ( 61.8 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
PKLR transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PKLR full-length
Host
Human
Theoretical MW (kDa)
63.25
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PKLR antibody (H00005313-B01) by Western Blots.
Western Blot
Lane 1: PKLR transfected lysate ( 61.8 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
PKLR transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PKLR
Entrez GeneID
5313GeneBank Accession#
NM_000298.4Protein Accession#
-Gene Name
PKLR
Gene Alias
PK1, PKL, PKR, PKRL, RPK
Gene Description
pyruvate kinase, liver and RBC
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
R-type/L-type pyruvate kinase|pyruvate kinase 1|pyruvate kinase isozyme R/L|pyruvate kinase type L|pyruvate kinase, liver and RBC type|pyruvate kinase, liver and blood cell|red cell/liver pyruvate kinase
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Interactome
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Pathway
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Disease
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