PKLR (Human) IP-WB Antibody Pair
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More Files
- More Functions
Immunoprecipitation of PKLR transfected lysate using rabbit polyclonal anti-PKLR and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-PKLR.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of PKLR transfected lysate using rabbit polyclonal anti-PKLR and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-PKLR.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-PKLR (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-PKLR (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — PKLR
Entrez GeneID
5313Gene Name
PKLR
Gene Alias
PK1, PKL, PKR, PKRL, RPK
Gene Description
pyruvate kinase, liver and RBC
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
R-type/L-type pyruvate kinase|pyruvate kinase 1|pyruvate kinase isozyme R/L|pyruvate kinase type L|pyruvate kinase, liver and RBC type|pyruvate kinase, liver and blood cell|red cell/liver pyruvate kinase
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Interactome
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Pathway
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Disease
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