PIP5K2A monoclonal antibody (M01), clone 3A3
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PIP5K2A.
Immunogen
PIP5K2A (NP_005019, 304 a.a. ~ 365 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYD
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (32.56 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PIP5K2A monoclonal antibody (M01), clone 3A3 Western Blot analysis of PIP5K2A expression in K-562 ( Cat # L009V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PIP5K2A on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PIP5K2A is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — PIP4K2A
Entrez GeneID
5305GeneBank Accession#
NM_005028Protein Accession#
NP_005019Gene Name
PIP4K2A
Gene Alias
FLJ13267, PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK
Gene Description
phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Omim ID
603140Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq
Other Designations
1-phosphatidylinositol-4-phosphate kinase|1-phosphatidylinositol-4-phosphate-5-kinase|OTTHUMP00000019300|OTTHUMP00000043353|PIP5KIIalpha|PtdIns(4)P-5-kinase B isoform|diphosphoinositide kinase|phosphatidylinositol-4-phosphate 5-kinase, type II, alpha|type
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