PIM1 monoclonal antibody (M08), clone 1C10
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PIM1.
Immunogen
PIM1 (AAH20224.1, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PIM1 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PIM1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PIM1
Entrez GeneID
5292GeneBank Accession#
BC020224.1Protein Accession#
AAH20224.1Gene Name
PIM1
Gene Alias
PIM
Gene Description
pim-1 oncogene
Omim ID
164960Gene Ontology
HyperlinkGene Summary
The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001 [PubMed 11518967]).[supplied by OMIM
Other Designations
OTTHUMP00000018046|Oncogene PIM1|non-specific serine/threonine protein kinase|pim-1 kinase 44 kDa isoform|pim-1 oncogene (proviral integration site 1)
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Interactome
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Pathway
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Disease
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Publication Reference
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The transcription factor STAT5 catalyzes Mannich ligation reactions yielding inhibitors of leukemic cell proliferation.
Wong EL, Nawrotzky E, Arkona C, Kim BG, Beligny S, Wang X, Wagner S, Lisurek M, Carstanjen D, Rademann J.
Nature Communications 2019 Jan; 10(1):66.
Application:WB, Mouse, BaF3/FLT3-ITD cells.
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The transcription factor STAT5 catalyzes Mannich ligation reactions yielding inhibitors of leukemic cell proliferation.
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