PIGA (Human) Recombinant Protein (Q01)
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More Files
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Specification
Product Description
Human PIGA partial ORF ( NP_002632, 194 a.a. - 293 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
LRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHDRVRLLGALEHK
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
36.74
Interspecies Antigen Sequence
Mouse (92); Rat (89)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — PIGA
Entrez GeneID
5277GeneBank Accession#
NM_002641Protein Accession#
NP_002632Gene Name
PIGA
Gene Alias
GPI3, PIG-A
Gene Description
phosphatidylinositol glycan anchor biosynthesis, class A
Omim ID
311770Gene Ontology
HyperlinkGene Summary
This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. [provided by RefSeq
Other Designations
GLCNAC-PI synthesis protein|GPI anchor biosynthesis|OTTHUMP00000022959|phosphatidylinositol N-acetylglucosaminyltransferase subunit A|phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria)|phosphatidylinositol-glycan biosynthesis, clas
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Interactome
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Pathway
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