PHEX 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PHEX transfected lysate.
Western Blot
Lane 1: PHEX transfected lysate ( 86.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PHEX full-length
Host
Human
Theoretical MW (kDa)
86.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PHEXantibody (H00005251-D01P)byWesternBlots.
SDS-PAGE Gel
PHEX transfected lysate.
Western Blot
Lane 1: PHEX transfected lysate ( 86.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PHEX
Entrez GeneID
5251GeneBank Accession#
NM_000444.3Protein Accession#
NP_000435.3Gene Name
PHEX
Gene Alias
HPDR, HPDR1, HYP, HYP1, PEX, XLH
Gene Description
phosphate regulating endopeptidase homolog, X-linked
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. [provided by RefSeq
Other Designations
OTTHUMP00000023051|phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets)|phosphate-regulating neutral endopeptidase
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Interactome
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Disease
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