PGM1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PGM1 full-length
Host
Human
Theoretical MW (kDa)
61.4
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PGM1 antibody (H00005236-D01P) by Western Blots.
SDS-PAGE Gel
PGM1 transfected lysate.
Western Blot
Lane 1: PGM1 transfected lysate ( 61.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PGM1
Entrez GeneID
5236GeneBank Accession#
NM_002633.2Protein Accession#
NP_002624.2Gene Name
PGM1
Gene Alias
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Gene Description
phosphoglucomutase 1
Omim ID
171900Gene Ontology
HyperlinkGene Summary
Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 [PubMed 8257433]).[supplied by OMIM
Other Designations
OTTHUMP00000010519|OTTHUMP00000046842
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