PGGT1B monoclonal antibody (M02), clone 5E4
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant PGGT1B.
Immunogen
PGGT1B (NP_005014, 1 a.a. ~ 105 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVATEDERLAGSGEGERLDFLRDRHVRFFQRCLQVLPERYSSLETSRLTIAFFALSGLDMLDSLDVVNKDDIIEWIYSLQVLPTEDRSNLNRCGFRGSSYLGIPF
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.29 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PGGT1B monoclonal antibody (M02), clone 5E4 Western Blot analysis of PGGT1B expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
PGGT1B monoclonal antibody (M02), clone 5E4. Western Blot analysis of PGGT1B expression in PC-12 ( Cat # L012V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PGGT1B is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — PGGT1B
Entrez GeneID
5229GeneBank Accession#
NM_005023Protein Accession#
NP_005014Gene Name
PGGT1B
Gene Alias
BGGI, GGTI
Gene Description
protein geranylgeranyltransferase type I, beta subunit
Omim ID
602031Gene Ontology
HyperlinkGene Summary
O
Other Designations
geranylgeranyltransferase type I beta subunit
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Interactomes
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Diseases
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Publication Reference
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GGTase3 is a newly identified geranylgeranyltransferase targeting a ubiquitin ligase.
Kuchay S, Wang H, Marzio A, Jain K, Homer H, Fehrenbacher N, Philips MR, Zheng N, Pagano M.
Nature Structural & Molecular Biology 2019 Jul; 26(7):628.
Application:WB-Tr, Human, HEK 293T, HeLa cells.
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Genetic studies on the functional relevance of the protein prenyltransferases in skin keratinocytes.
Lee R, Chang SY, Trinh H, Tu Y, White AC, Davies BS, Bergo MO, Fong LG, Lowry WE, Young SG.
Human Molecular Genetics 2010 Apr; 19(8):1603.
Application:WB-Ce, Mouse, Skin keratinocytes.
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GGTase3 is a newly identified geranylgeranyltransferase targeting a ubiquitin ligase.
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