PFKL 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PFKL full-length
Host
Human
Theoretical MW (kDa)
91.08
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PFKL antibody (H00005211-B01) by Western Blots.
SDS-PAGE Gel
PFKL transfected lysate.
Western Blot
Lane 1: PFKL transfected lysate ( 91.08 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PFKL
Entrez GeneID
5211GeneBank Accession#
NM_001002021.1Protein Accession#
NP_001002021.1Gene Name
PFKL
Gene Alias
DKFZp686G1648, DKFZp686L2097, FLJ30173, FLJ40909, PFK-B
Gene Description
phosphofructokinase, liver
Omim ID
171860Gene Ontology
HyperlinkGene Summary
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq
Other Designations
6-phosphofructokinase, liver type|liver phosphofructokinase|liver-type 1-phosphofructokinase|phosphofructo-1-kinase isozyme B|phosphofructokinase 1|phosphohexokinase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
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