PFKFB2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
PFKFB2 transfected lysate.
Western Blot
Lane 1: PFKFB2 transfected lysate ( 55.66 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PFKFB2 full-length
Host
Human
Theoretical MW (kDa)
55.66
Interspecies Antigen Sequence
Mouse (93); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PFKFB2 antibody (H00005208-B01) by Western Blots.
SDS-PAGE Gel
PFKFB2 transfected lysate.
Western Blot
Lane 1: PFKFB2 transfected lysate ( 55.66 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PFKFB2
Entrez GeneID
5208GeneBank Accession#
NM_006212.2Protein Accession#
NP_006203.2Gene Name
PFKFB2
Gene Alias
DKFZp781D2217, MGC138308, MGC138310, PFK-2/FBPase-2
Gene Description
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Omim ID
171835Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2|6PF-2-K/Fru-2,6-P2ASE heart-type isozyme|OTTHUMP00000034285|OTTHUMP00000034286|PFKFB, cardiac|fructose-2,6-bisphosphatase, cardiac isozyme
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Interactome
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Disease
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