PFKFB1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PFKFB1 transfected lysate.
Western Blot
Lane 1: PFKFB1 transfected lysate ( 51.92 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PFKFB1 full-length
Host
Human
Theoretical MW (kDa)
51.92
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PFKFB1 antibody (H00005207-B01) by Western Blots.
SDS-PAGE Gel
PFKFB1 transfected lysate.
Western Blot
Lane 1: PFKFB1 transfected lysate ( 51.92 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PFKFB1
Entrez GeneID
5207GeneBank Accession#
NM_002625.1Protein Accession#
NP_002616.1Gene Name
PFKFB1
Gene Alias
F6PK, HL2K, MGC116715, MGC116717, PFRX
Gene Description
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Omim ID
311790Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq
Other Designations
6PF-2-K/Fru-2,6-P2ASE liver isozyme|OTTHUMP00000023391|fructose-2,6-bisphosphatase|fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
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Interactome
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Disease
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