CFP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CFP transfected lysate.
Western Blot
Lane 1: CFP transfected lysate ( 51.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CFP full-length
Host
Human
Theoretical MW (kDa)
51.3
Interspecies Antigen Sequence
Mouse (77); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CFP antibody (H00005199-B01) by Western Blots.
SDS-PAGE Gel
CFP transfected lysate.
Western Blot
Lane 1: CFP transfected lysate ( 51.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CFP
Entrez GeneID
5199GeneBank Accession#
NM_002621Protein Accession#
NP_002612Gene Name
CFP
Gene Alias
BFD, PFC, PFD, PROPERDIN
Gene Description
complement factor properdin
Gene Ontology
HyperlinkGene Summary
This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified
Other Designations
properdin P factor, complement
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Interactome
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Disease
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