PEPD monoclonal antibody (M01), clone 1D5-H3
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More Files
- More Functions
Western Blot (Cell lysate)
PEPD monoclonal antibody (M01), clone 1D5-H3 Western Blot analysis of PEPD expression in HepG2 ( Cat # L019V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.
Lane 1: PEPD transfected lysate (Predicted MW: 54.6 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PEPD transfected lysate using anti-PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PEPD is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (79.97 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PEPD.
Immunogen
PEPD (AAH15027, 1 a.a. ~ 493 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVAEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQEPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (79.97 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PEPD monoclonal antibody (M01), clone 1D5-H3 Western Blot analysis of PEPD expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.
Lane 1: PEPD transfected lysate (Predicted MW: 54.6 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PEPD transfected lysate using anti-PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PEPD is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10 ug/ml] -
Gene Info — PEPD
Entrez GeneID
5184GeneBank Accession#
BC015027Protein Accession#
AAH15027Gene Name
PEPD
Gene Alias
MGC10905, PROLIDASE
Gene Description
peptidase D
Omim ID
170100Gene Ontology
HyperlinkGene Summary
Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of iminoacids. [provided by RefSeq
Other Designations
imidodipeptidase|proline dipeptidase
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Interactome
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Disease
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