PECAM1 monoclonal antibody (M01), clone 1D2-1A5
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More Files
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Western Blot (Tissue lysate)
PECAM1 monoclonal antibody (M01), clone 1D2-1A5. Western Blot analysis of PECAM1 expression in human placenta.
Western Blot (Transfected lysate)
Western Blot analysis of PECAM1 expression in transfected 293T cell line by PECAM1 monoclonal antibody (M01), clone 1D2-1A5.
Lane 1: PECAM1 transfected lysate(82.4 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PECAM1 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma tissue. [antibody concentration 5 ug/ml]
Immunoprecipitation
Immunoprecipitation of PECAM1 transfected lysate using anti-PECAM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PECAM1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PECAM1 is 0.1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (103.95 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PECAM1.
Immunogen
PECAM1 (AAH22512, 29 a.a. ~ 738 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (103.95 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
PECAM1 monoclonal antibody (M01), clone 1D2-1A5. Western Blot analysis of PECAM1 expression in human placenta.Western Blot (Transfected lysate)
Western Blot analysis of PECAM1 expression in transfected 293T cell line by PECAM1 monoclonal antibody (M01), clone 1D2-1A5.
Lane 1: PECAM1 transfected lysate(82.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PECAM1 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma tissue. [antibody concentration 5 ug/ml]Immunoprecipitation
Immunoprecipitation of PECAM1 transfected lysate using anti-PECAM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PECAM1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PECAM1 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — PECAM1
Entrez GeneID
5175GeneBank Accession#
BC022512Protein Accession#
AAH22512Gene Name
PECAM1
Gene Alias
CD31, PECAM-1
Gene Description
platelet/endothelial cell adhesion molecule
Omim ID
173445Gene Ontology
HyperlinkGene Summary
CD31/EndoCAM|adhesion molecule
Other Designations
CD31 antigen|CD31/EndoCAM|PECAM-1, CD31/EndoCAM|adhesion molecule
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Interactome
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Pathway
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Disease
- Acute Disease
- Angina Pectoris
- Arteriosclerosis
- Asthma
- Astrocytoma
- Atherosclerosis
- Brain Ischemia
- Bronchiolitis
- Cadaver
+ View More Disease
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Publication Reference
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Fluorescence-guided Fiber-Optic Micronavigation Using Microscopic Identification of Vascular Boundary of Liver Segment and Tumors.
Qingliang Wang, Baifeng Qian, Michael Schäfer, Wolfgang Groß, Arianeb Mehrabi, Eduard Ryschich.
Theranostics 2020 May; 10(14):6136.
Application：IF, IHC-Fr, Human, Human hepatocellular carcinoma, Human livers.
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DRG2 supports the growth of primary tumors and metastases of melanoma by enhancing VEGF-A expression.
Nal Ae Yoon, Se Jin Jung, Seong Hee Choi, Jin Hyun Ryu, Muralidharan Mani, Unn Hwa Lee, Mai-Tram Vo, Do Yong Jeon, Su Wol Chung, Byung Ju Lee, Young Wha Koh, Soon Eun Park, Yong Joon Shin, Sang Soo Kang, Wha Ja Cho, Hee Jeong Cha, Jeong Woo Park.
The FEBS Journal 2020 May; 287(10):2070.
Application：IHC-P, Mouse, B16F10 cells.
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Fluorescence-guided Fiber-Optic Micronavigation Using Microscopic Identification of Vascular Boundary of Liver Segment and Tumors.
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