PDPK1 monoclonal antibody (M05), clone 2E2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PDPK1.
Immunogen
PDPK1 (NP_002604, 457 a.a. ~ 556 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ
Host
Mouse
Reactivity
Human, Rat
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
PDPK1 monoclonal antibody (M05), clone 2E2. Western Blot analysis of PDPK1 expression in rat brain.Western Blot (Cell lysate)
PDPK1 monoclonal antibody (M05), clone 2E2. Western Blot analysis of PDPK1 expression in IMR-32.Western Blot (Transfected lysate)
Western Blot analysis of PDPK1 expression in transfected 293T cell line by PDPK1 monoclonal antibody (M05), clone 2E2.
Lane 1: PDPK1 transfected lysate (Predicted MW: 48.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — PDPK1
Entrez GeneID
5170GeneBank Accession#
NM_002613Protein Accession#
NP_002604Gene Name
PDPK1
Gene Alias
MGC20087, MGC35290, PDK1, PRO0461
Gene Description
3-phosphoinositide dependent protein kinase-1
Omim ID
605213Gene Ontology
HyperlinkOther Designations
PkB kinase like gene 1|PkB-like 1|protein kinase
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Interactome
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Pathway
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Disease
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