PDK4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PDK4 transfected lysate.
Western Blot
Lane 1: PDK4 transfected lysate ( 46.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDK4 full-length
Host
Human
Theoretical MW (kDa)
46.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDK4 antibody (H00005166-D01P) by Western Blots.
SDS-PAGE Gel
PDK4 transfected lysate.
Western Blot
Lane 1: PDK4 transfected lysate ( 46.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PDK4
Entrez GeneID
5166GeneBank Accession#
NM_002612Protein Accession#
NP_002603.1Gene Name
PDK4
Gene Alias
FLJ40832
Gene Description
pyruvate dehydrogenase kinase, isozyme 4
Omim ID
602527Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq
Other Designations
pyruvate dehydrogenase kinase 4|pyruvate dehydrogenase kinase, isoenzyme 4
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