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Last updated: 2017/5/28

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PDK1 rabbit monoclonal antibody

  • Catalog # : H00005163-K
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against a human PDK1 peptide using ARM Technology.
  • Immunogen:
  • A synthetic peptide of human PDK1 is used for rabbit immunization.
    Customer or Abnova will decide on the preferred peptide sequence.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Purification:
  • Protein A
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody reactive against human PDK1 peptide by ELISA and mammalian transfected lysate by Western Blot.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 1. Customer may provide cell or tissue lysate for antibody screening.
    2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request.
  • Library Construction:
  • Non-fusion antibody library from rabbit spleen (ARM Technology).
  • Expression:
  • Overexpression vector and transfection into 293H cell line.
  • Deliverable:
  • Up to three rabbit IgG clones of 1 mg each will be delivered to customer.
  • Applications
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5163
  • GeneBank Accession#:
  • PDK1
  • Gene Name:
  • PDK1
  • Gene Alias:
  • -
  • Gene Description:
  • pyruvate dehydrogenase kinase, isozyme 1
  • Gene Summary:
  • Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq
  • Other Designations:
  • mitochondrial pyruvate dehydrogenase kinase isoenzyme 1,pyruvate dehydrogenase kinase, isoenzyme 1
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