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PDHA1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005160-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PDHA1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 42.64
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PDHA1 antibody (H00005160-B01) by Western Blots.
    Western Blot
    QC Testing of H00005160-T01
    Lane 1: PDHA1 transfected lysate ( 43.01 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00005160-T01
    PDHA1 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5160
  • Gene Name:
  • PDHA1
  • Gene Alias:
  • PDHA,PDHCE1A,PHE1A
  • Gene Description:
  • pyruvate dehydrogenase (lipoamide) alpha 1
  • Gene Summary:
  • The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000023015,pyruvate dehydrogenase E1 alpha subunit
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