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PDGFA 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005154-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PDGFA full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 24
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PDGFA antibody (H00005154-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005154-T01
    PDGFA transfected lysate
    Western Blot
    QC Testing of H00005154-T01
    Lane 1: PDGFA transfected lysate ( 24 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5154
  • Gene Name:
  • PDGFA
  • Gene Alias:
  • PDGF-A,PDGF1
  • Gene Description:
  • platelet-derived growth factor alpha polypeptide
  • Gene Summary:
  • The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • PDGF A-chain,platelet-derived growth factor alpha,platelet-derived growth factor alpha chain,platelet-derived growth factor alpha isoform 2 preproprotein
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