PDGFA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PDGFA transfected lysate
Western Blot
Lane 1: PDGFA transfected lysate ( 24 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDGFA full-length
Host
Human
Theoretical MW (kDa)
24
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDGFA antibody (H00005154-B01) by Western Blots.
SDS-PAGE Gel
PDGFA transfected lysate
Western Blot
Lane 1: PDGFA transfected lysate ( 24 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PDGFA
Entrez GeneID
5154GeneBank Accession#
NM_002607Protein Accession#
NP_002598Gene Name
PDGFA
Gene Alias
PDGF-A, PDGF1
Gene Description
platelet-derived growth factor alpha polypeptide
Omim ID
173430Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq
Other Designations
PDGF A-chain|platelet-derived growth factor alpha|platelet-derived growth factor alpha chain|platelet-derived growth factor alpha isoform 2 preproprotein
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Interactome
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Pathway
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Disease
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