PCOLCE 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PCOLCE full-length
Host
Human
Theoretical MW (kDa)
48
Interspecies Antigen Sequence
Mouse (80); Rat (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PCOLCE antibody (H00005118-B01P) by Western Blots.
SDS-PAGE Gel
PCOLCE transfected lysate.
Western Blot
Lane 1: PCOLCE transfected lysate ( 48.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PCOLCE
Entrez GeneID
5118GeneBank Accession#
BC000574.2Protein Accession#
AAH00574.1Gene Name
PCOLCE
Gene Alias
PCPE, PCPE1
Gene Description
procollagen C-endopeptidase enhancer
Omim ID
600270Gene Ontology
HyperlinkGene Summary
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq
Other Designations
procollagen C-proteinase enhancer 1|procollagen, type 1, COOH-terminal proteinase enhancer
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