PCK2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PCK2 transfected lysate
Western Blot
Lane 1: PCK2 transfected lysate ( 70.7 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PCK2 full-length
Host
Human
Theoretical MW (kDa)
70.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PCK2 antibody (H00005106-B01) by Western Blots.
SDS-PAGE Gel
PCK2 transfected lysate
Western Blot
Lane 1: PCK2 transfected lysate ( 70.7 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PCK2
Entrez GeneID
5106GeneBank Accession#
BC001454Protein Accession#
AAH01454Gene Name
PCK2
Gene Alias
PEPCK, PEPCK-M, PEPCK2
Gene Description
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Omim ID
261650Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
OTTHUMP00000164700|PEP carboxykinase|mitochondrial phosphoenolpyruvate carboxykinase 2|phosphoenolpyruvate carboxylase|phosphopyruvate carboxylase
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