PCK2 rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human PCK2 peptide using ARM Technology.
Immunogen
A synthetic peptide of human PCK2 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human PCK2 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — PCK2
Entrez GeneID
5106GeneBank Accession#
PCK2Gene Name
PCK2
Gene Alias
PEPCK, PEPCK-M, PEPCK2
Gene Description
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Omim ID
261650Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
OTTHUMP00000164700|PEP carboxykinase|mitochondrial phosphoenolpyruvate carboxykinase 2|phosphoenolpyruvate carboxylase|phosphopyruvate carboxylase
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